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Furthermore, FF from PCOS patients is characterized by an excess of ROS and NOX2, a subunit of NADPH oxidase that generates superoxide radicals, contributing to altered follicular signaling [71,72,73,74]

Briefly, samples were deproteinized by the addition of sulfosalicylic acid, were then incubated for 30 min at 4C, and subsequently centrifuged for 20 minutes at 12,000 g