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PMID: 20190196 Reported Macrocycle stability Lactam-bridged backbone confers proteolytic stability The side-chaintoside-chain lactam bridge between Asp(2) and Lys(7) is reported in macrocyclization-strategies reviews to confer proteolytic stability relative to linear -MSH and to constrain the receptor-binding pharmacophore (the conserved His-Phe-Arg-Trp / HFRW core, with D-Phe at position 4 of the cycle in PT-141)

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This enzyme catalyzes the transfer of the -glutamyl moiety of glutathione (GSH) to another amino acid to produce a -Glu-AA and cysteinyl-glycine

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M5655) performed as per manufacturers instructions to quantify viable cells as a proxy of proliferation relative to the control